- Bone cell culture model

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	Biocompatible Materials » Project survey » Exploratory projects » Bone cell culture model

Background and description

This project was a collaboration between Dept. of Handicap research (HKF) at Göteborg University and IFM at Linköping University. The goals of the project were to prepare a bioactive functionalized implant surface that shows: (i) a thinner fibrous tissue formation around implants and (ii) a quick functional integration to tissues, initially to bone. Cell attachment and spreading, the expression of phenotype, the production of mineralized deposits and gene expression (DNA-arrays available via SWEGENE) were used to evaluate the bone cell response.

Scientific results

Surface evaluation revealed similar roughness for the turned control and the anodised surface with Mg²⁺ ions incorporated, while the blasted surface demonstrated a rougher surface profile.

LDH (lactatdehydrogenas) values were generally low for all surfaces (within the range of 0.8-1.6 μkat/l) but were slightly increased after LPS stimulation and after 72h. TNF-α was transiently higher day one and after LPS stimulation, especially on the turned control surface. Further, IL-10 levels were generally low irrespective of time. Increased IL-10 amounts after LPS stimulation and after 24h were observed for all surfaces. The total cell numbers decreased on all surfaces from 24h to 72h but there were no major difference between stimulated and non-stimulated wells. Acute monocytic phenotype 27E10 marker dominated on all surfaces while the expression of the chronic RM3/1 marker was almost absent on all surfaces both at 24h and 72h.

Conclusion: The present study indicates a surface topography and chemistry related difference in the acute inflammatory response with a stronger acute inflammatory response to the turned control compared to the blasted and the anodised surface with Mg²⁺ ions incorporated. The project will continue until the end of year 2004.

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