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Transmission electron microscopy (TEM)
Optical microscopes use light to produce an image. Diffraction of
the incident light limits the resolution of the microscope, so that the smallest
things we can observe are in the same size regime as the wavelength of visible
light, which spans from about 380 to 780 nm. In order to see smaller things
than this, we have to use some other technique.
Thanks to the duality of quantum physics, which states that any particle,
including an electron, can have both wave and particle properties, electrons
can be used instead of photons to produce an image. Since electrons can have much
higher energy than photons, they can also have much smaller wavelengths. The
resolution can therefore be drastically improved compared to that of
an optical microscope.
In a transmission electron microsope (TEM) a beam of electrons is emitted from
an electron gun. The electrons are then accelerated to an energy of 100-200 keV
(compared to a few eV for photons). The beam is refined and lead to the specimen
via an array of magnetic lenses and is transmitted through the sample.
The beam then creates an image of the specimen, or a diffraction pattern,
after another set of lenses.
We have two TEMs:
Philips CM200
JEOL 2000FX
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Jan 1S52  2M1 3T 4O 5T 6F 7L 8S  9M2 10T 11O 12T 13F 14L 15S  16M3 17T 18O 19T 20F 21L 22S  23M4 24T 25O 26T 27F 28L 29S  30M5 31T |
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Feb 1O 2T 3F 4L 5S  6M6 7T 8O 9T 10F 11L 12S  13M7 14T 15O 16T 17F 18L 19S  20M8 21T 22O 23T 24F 25L 26S  27M9 28T 29O |
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Mar 1T 2F 3L 4S  5M10 6T 7O 8T 9F 10L 11S  12M11 13T 14O 15T 16F 17L 18S  19M12 20T 21O 22T 23F 24L 25S  26M13 27T 28O 29T 30F 31L |
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